Supported Membrane Templates (SMrT) are a versatile assay system that display membranes of different shapes - from planar patches to curved nanotubes. Our workflow simply involves screening for membrane integrity disrupting (MID) proteins in cell and tissue lysates by flowing them on SMrTs. MID activity manifests either as budding or tubulation of the planar membrane or fission of membrane nanotubes seen using fluorescence microscopy (see movies). We then identify the active protein using biochemical fractionation followed by mass spectrometry and following this up with an in-depth understanding of their physiological functions using appropriate model organisms.