Preprints 

 

• Division-arrest induced filamentation protects uropathogenic Escherichia coli from killing by the cathelicidin antimicrobial peptide LL-37. Atakgayeva G, Porco N, Rudovskaya S, Sarkar M, Tomlinson M, Huynh R, Pucadyil T, Botelho R, McPhee JB. bioarxiv

• Membrane remodeling by the Bacterial Dynamin-like Protein (BDLP) from Nostoc punctiforme. Singh K, Pucadyil TJ. bioarxiv

• Vacuolar protein sorting-associated protein 1 (Vps1) has membrane constricting and severing abilities required for endosomal protein trafficking. Gopan S, Singh G, Swaminathan U, Pucadyil TJ. bioarxiv

2025

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• Division of labor among fission dynamins based on substrate size. Sarkar M, Pucadyil TJ Biochemistry

• Wag31, a membrane tether, is crucial for lipid homeostasis in mycobacteria. Kapoor Y, Khurana H, Dutta D, Chakraborty A, Priya A, Singh A, Kamat SS, Dhar N, Pucadyil TJ, Nandicoori VK. Elife 

• Acute diacylglycerol production activates critical membrane-shaping proteins leading to mitochondrial tubulation and fission. Pemberton JG, Roy K, Kim YJ, Fischer TD, Joshi V, Ferrer E, Youle RJ, Pucadyil TJ, Balla T. Nature Communications 

2024
 

• Dynamics of membrane tubulation coupled with fission by a two-component module. Bhattacharyya S, Pucadyil TJ. Proceedings of the National Academy of Sciences USA

• The race to uncover fission factors for lysosomal organelles heats up. Gopan S, Pucadyil TJ. Nature  

• Reconstituting membrane fission using a high content and throughput assay. Swaminathan U, Pucadyil TJ. Biochemical Society Transactions
   

2023
 

• 'Gearing' up for dynamin-catalyzed membrane fission. Khurana H, Pucadyil TJ. Current Opinions in Cell Biology 

• Membrane contacts, lipid flux, and fission. Pucadyil TJ Molecular Cell

• Insights into cargo sorting by SNX32 and its role in neurite outgrowth. Sugatha J, Priya A, Raj P, Jaimon E, Swaminathan U, Jose A, Pucadyil TJ, Datta S. Elife 

• Mechanistic analysis of a novel membrane-interacting variable loop in the pleckstrin-homology domain critical for dynamin function. Khurana H, Baratam K, Bhattacharyya S, Srivastava A, Pucadyil TJ. Proceedings of the National Academy of Sciences USA
   

2022
 

• Is Drp1 sufficient to catalyze membrane fission? Roy K, Pucadyil TJ. Proceedings of the National Academy of Sciences USA 

• Metal-binding propensity in the mitochondrial dynamin-related protein. Roy K, Pucadyil TJ. Journal of Membrane Biology 

• Protein-protein interactions on membrane surfaces analysed using pull downs with supported bilayers on silica beads. Andhare DS, Khurana H, Pucadyil TJ. Journal of Membrane Biology  
   

2021
 

• Function and regulation of the divisome for mitochondrial fission. Krauss F, Roy K, Pucadyil TJ, Ryan MJ. Nature

• Understanding membrane traffic from molecular ensemble, energetics, and the cell biology of participant components. Klumperman J, Pucadyil T. Current Opinions in Cell Biology

• Molecular Interplay at the Membrane and Impact on Cellular Physiology. Pucadyil T, Kulkarni K, Sengupta D.
  Journal of Membrane Biology

 

2020
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• PLiMAP: Proximity-Based Labeling of Membrane-Associated Proteins. Jose GP, Pucadyil TJ. Current Protocols in Protein Sciences

• Cellular functions and intrinsic attributes of the ATP-binding Eps15 homology domain-containing proteins. Bhattacharyya S, Pucadyil TJ. Protein Science

• A facile, sensitive and quantitative membrane-binding assay for proteins. Jose GP, Gopan S, Bhattacharyya S, Pucadyil TJ Traffic 

2019
 

• A screen for membrane fission catalysts identifies the ATPase EHD1. Kamerkar S, Roy K, Bhattacharyya S, Pucadyil TJ. Biochemistry 
   

2018
 

• Dynamin-related protein 1 has membrane constricting and severing abilities sufficient for mitochondrial and peroxisomal fission. Kamerkar SC, Kraus F, Sharpe A, Pucadyil TJ, Ryan MT Nature Communications

• ATP-dependent membrane remodeling links EHD1 functions to endocytic recycling. Deo R, Kushwah MS, Kamerkar SC, Kadam NY, Dar S, Babu K, Srivastava A, Pucadyil TJ. Nature Communications 

• Salmonella SipA mimics a cognate SNARE for host Syntaxin8 to promote fusion with early endosomes. Singh PK, Kapoor A, Lomash RM, Kumar K, Kamerkar SC, Pucadyil TJ, Mukhopadhyay A. Journal of Cell Biology

• SMrT Assay for Real-Time Visualization and Analysis of Clathrin Assembly Reactions. Andhare D, Holkar SS, Pucadyil TJ. Methods in Molecular Biology

• The 2018 biomembrane curvature and remodeling roadmap. Bassereau P, Jin R, Baumgart T, Deserno M, Dimova R, Frolov VA, Bashkirov PV, Grubmüller H, Jahn R, Risselada HJ, Johannes L, Kozlov MM, Lipowsky T, Pucadyil TJ, Zeno WF, Stachowiak JC, Stamou D, Breuer A, Lauritsen L, Simon C, Sykes C, Voth GA, Weikl TR Journal of Physics D: Applied Physics

• A novel fluorescence microscopic approach to quantitatively analyse protein-induced membrane remodelling. Pucadyil TJ Journal of Bioscience

2017
 

• Excess area dependent scaling behavior of nano-sized membrane tethers. Natesan R, Sreeja KK, Roychoudhuri A, Eckmann DM, Ayyaswamy PS, Baumgart T, Pucadyil T, Patil SV, Weaver VM, Radhakrishnan R. Physical Biology

• The pleckstrin-homology domain of dynamin is dispensable for membrane constriction and fission. Dar S, Pucadyil TJ. Molecular Biology of the Cell

• Use of the supported membrane tube assay system for real-time analysis of membrane fission reactions. Dar S, Kamerkar SC, Pucadyil TJ. Nature Protocols

• Sphingolipids modulate the function of human serotonin1A receptors: Insights from sphingolipid-deficient cells.
  Jafurulla M, Bandari S, Pucadyil TJ, Chattopadhyay A. Biochimica et Biophys Acta Biomembranes

 

2016​

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• Comparative analysis of adaptor-mediated clathrin assembly reveals general principles for adaptor clustering. Pucadyil TJ, Holkar SS. Molecular Biology of the Cell

2015
 

• A high-throughput platform for real-time analysis of membrane fission reactions reveals the mechanism of dynamin function. Dar S, Kamerkar SC, Pucadyil TJ. Nature Cell Biology

• Spatial control of epsin-induced clathrin assembly by membrane curvature. Holkar SS, Kamerkar SC, Pucadyil TJ.  Journal of Biological Chemistry

• Thomas Pucadyil: Piecing together membrane fission. Pucadyil T, Sedwick C. Journal of Cell Biology
   

2013
 

• Geometric catalysis of membrane fission driven by flexible dynamin rings. Shnyrova AV, Bashkirov PV, Akimov SA, Pucadyil TJ, Zimmerberg J, Schmid SL, Frolov VA. Science 

• Analyzing membrane remodeling and fission using supported bilayers with excess membrane reservoir. Neumann S, Pucadyil TJ, Schmid SL. Nature Protocols

2011

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• Differential curvature sensing and generating activities of dynamin isoforms provide opportunities for tissue-specific regulation. Liu Y-W, Neumann S, Ramachandran R, Ferguson SM, Pucadyil TJ, Schmid SL. Proceedings of the National Academy USA

• Dynamic remodeling of membranes catalyzed by dynamin. Pucadyil TJ. Current Topics in Membranes
   

2010

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• Supported bilayers with excess membrane reservoir (SUPER): A template for reconstituting membrane budding and fission. Pucadyil TJ, Schmid SL. Biophysical Journal

• Chronic cholesterol depletion using statin impairs the function and dynamics of human serotonin(1A) receptors.
  Shrivastava S, Pucadyil TJ, Paila YD, Ganguly S, Chattopadhyay A. Biochemistry

 

2009

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• Dissecting dynamin's role in clathrin-mediated endocytosis. Mettlen M, Pucadyil TJ, Ramachandran R, Schmid SL. Biochemical Society Transactions

• Conserved functions of Membrane Active GTPases in Coated Vesicle Formation. Pucadyil TJ, Schmid SL. Science

• Membrane insertion of the pleckstrin homology domain variable loop 1 is Critical for Dynamin-catalyzed Vesicle Scission. Ramachandran R, Pucadyil TJ, Liu Y-W, Acharya S, Leonard M, Lukiyanchuk V, Schmid SL. Molecular Biology of the Cell

• An Intramolecular Signaling Element that Modulates Dynamin Function In Vitro and In Vivo. Chappie, J.S., Acharya, S., Liu, Y.-W., Leonard, M., Pucadyil, T.J., Schmid, S.L. Molecular Biology of the Cell 

 

2008

 

• Real-time visualization of dynamin-catalyzed membrane fission and vesicle release. Pucadyil TJ, Schmid SL Cell

• Effect of sphingomyelinase treatment on ligand binding activity of human serotonin1A receptors. Jafurulla M, Pucadyil TJ, Chattopadhyay A. Biochimica et Biophys Acta Biomembranes

• Actin cytoskeleton-dependent dynamics of the human serotonin1A receptor correlates with receptor signaling.
  Ganguly S, Pucadyil TJ, Chattopadhyay A. Biophysical Journal
   

2007

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• Cholesterol depletion induces dynamic confinement of the G-protein coupled serotonin(1A) receptor in the plasma membrane of living cells. Pucadyil TJ, Chattopadhyay A. Biochimica et Biophysica Acta Biomembranes

• Membrane Organization and Dynamics of the Serotonin1A Receptor Monitored Using Fluorescence Microscopic Approaches. Kalipatnapu S, Pucadyil TJ, Chattopadhyay A. Serotonin Receptors in Neurobiology

• Cholesterol: a potential therapeutic target in Leishmania infection? Pucadyil TJ, Chattopadhyay A. Trends in Parasitology

• The human serotonin1A receptor exhibits G-protein-dependent cell surface dynamics. Pucadyil TJ, Chattopadhyay A. Glycoconjugate Journal

• Organization and dynamics of NBD-labeled lipids in membranes analyzed by fluorescence recovery after photobleaching. Pucadyil TJ, Mukherjee S, Chattopadhyay A. Journal of Physical Chemistry B

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2006

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• Monitoring the organization and dynamics of bovine hippocampal membranes utilizing differentially localized fluorescent membrane probes. Mukherjee S, Kalipatnapu S, Pucadyil TJ, Chattopadhyay A. Molecular Membrane Biology

• Prolonged treatment with ligands affects ligand binding to the human serotonin(1A) receptor in Chinese hamster ovary cells. Pucadyil TJ, Jafurulla M, Chattopadhyay A. Cellular and Molecular Neurobiology

• Ligand binding and G-protein coupling of the serotonin1A receptor in cholesterol-enriched hippocampal membranes.
  Chattopadhyay A, Jafurulla M, Pucadyil TJ. Bioscience Reports

• Role of cholesterol in the function and organization of G-protein coupled receptors. Pucadyil TJ, Chattopadhyay A. Progress in Lipid Research

• Confocal fluorescence recovery after photobleaching of green fluorescent protein in solution. Pucadyil TJ, Chattopadhyay A. Journal of Fluorescence

• Effect of cholesterol on lateral diffusion of fluorescent lipid probes in native hippocampal membranes. Pucadyil TJ, Chattopadhyay A. Chemistry and Physics of Lipids

• The sterol-binding antibiotic nystatin inhibits entry of non-opsonized Leishmania donovani into macrophages.
  Tewary P, Veena K, Pucadyil TJ, Chattopadhyay A, Madhubala R. Biochemical and Biophysical Research Communications

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2005

 

• Membrane organization and dynamics of the G-protein-coupled serotonin1A receptor monitored using fluorescence-based approaches. Pucadyil TJ, Kalipatnapu S, Chattopadhyay A. Journal of Fluorescence

• The cholesterol-complexing agent digitonin modulates ligand binding of the bovine hippocampal serotonin 1A receptor. Paila YD, Pucadyil TJ, Chattopadhyay A. Molecular Membrane Biology

• The serotonin1A receptor: a representative member of the serotonin receptor family. Pucadyil TJ, Kalipatnapu S, Chattopadhyay A. Cellular and Molecular Neurobiology

• Cholesterol modulates the antagonist-binding function of hippocampal serotonin1A receptors. Pucadyil TJ, Chattopadhyay A. Biochimica et Biophysica Acta Biomembrane

• Membrane cholesterol oxidation inhibits ligand binding function of hippocampal serotonin(1A) receptors. Pucadyil TJ, Shrivastava S, Chattopadhyay A. Biochemical and Biophysical Research Communications

• Role of cholesterol in ligand binding and G-protein coupling of serotonin1A receptors solubilized from bovine hippocampus. Chattopadhyay A, Jafurulla M, Kalipatnapu S, Pucadyil TJ, Harikumar KG. Biochemical and Biophysical Research Communications

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2004

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• Ligand binding characteristics of the human serotonin1A receptor heterologously expressed in CHO cells. Kalipatnapu S, Pucadyil TJ, Harikumar KG, Chattopadhyay A. Bioscience Reports

• G-protein-dependent cell surface dynamics of the human serotonin1A receptor tagged to yellow fluorescent protein. Pucadyil TJ, Kalipatnapu S, Harikumar KG, Rangaraj N, Karnik SS, Chattopadhyay A. Biochemistry

• The sterol-binding antibiotic nystatin differentially modulates ligand binding of the bovine hippocampal serotonin1A receptor. Pucadyil TJ, Shrivastava S, Chattopadhyay A. Biochemical and Biophysical Research Communications

• Cholesterol modulates ligand binding and G-protein coupling to serotonin(1A) receptors from bovine hippocampus. Pucadyil TJ, Chattopadhyay A. Biochimica et Biophysica Acta Biomembrane

• Membrane sphingolipid-ergosterol interactions are important determinants of multidrug resistance in Candida albicans. Mukhopadhyay K, Prasad T, Saini P, Pucadyil TJ, Chattopadhyay A, Prasad R. Antimicrobial Agents and Chemotherapy

• Exploring detergent insolubility in bovine hippocampal membranes: a critical assessment of the requirement for cholesterol. Pucadyil TJ, Chattopadhyay A. Biochimica et Biophysica Acta Biomembranes

• Cholesterol is required for Leishmania donovani infection: implications in leishmaniasis. Pucadyil TJ, Tewary P, Madhubala R, Chattopadhyay A. Molecular Biochemical Parasitology